Ameliorating Effect of Tinospora Cordifolia Leaf Extract Loaded Phytoniosome on Mifepristone Induced PCOS Rats – A Biochemical Study

Abstract

The most common endocrinological reproductive disorder in women is Polycystic Ovarian Syndrome (PCOS).The herbTinospora cordifolia, (Menispermaceae) is a famous medicinal plant which hashypoglycaemic effect.Niosomes are considered as novel drug carriers in drug delivery systems with bilayer assembly made up of self-assembled structures of cholesterol and non-ionic surfactants using aqueous phase. Thisstudy is performed to assess the ameliorative effect of ethanolic leaf extract ofTinospora cordifolia(TCELE) and ethanolic leaf extract ofTinospora cordifolia loaded phytoniosome (pnTCELE) on PCOS in mifepristone induced female rats. For a period of 8 days PCOS was induced by administrating of mifepristone daily to the female rats which was confirmedby persistent estrous cycle. 200mg /kg b. w of TCELE and pnTCELE extracts were given to the rats which attained PCOS condition for 28 days.Weight of the reproductive organs and the body weight were analysed after treatment along with estimation of biochemical parameters. Metformin was used as positive control.The body weight and weight of the reproductive organs were increased significantly. Additionally significant dyslipidaemiaand hyperglycaemiawere seen in PCOS rats which were normalized in pnTCELE drug treated animals and TCELE drug treated animals.Thus, pnTCELE can be effectively used for the supervision of PCOS which requires a control of endocrine and metabolic complication