Anti-Oxidant and Anti-Inflammarory Activity on Aqueous Solution Vs Methonolic Extract of Annona Muricata

Abstract

Background:
Annona muricata, known as soursop or graviola, is a tropical fruit-bearing tree from the Caribbean, Central America, and South America. It is in the Annonaceae family and has been used medicinally in many cultures. Folk medicine uses the plant's leaves, fruit, seeds, and bark to cure fever, pain, respiratory difficulties, and infections. An imbalance between free radicals and antioxidants in the body causes oxidative stress, which contributes to chronic diseases like cancer, cardiovascular disease, and neurological disorders. By neutralizing free radicals, antioxidants protect cells from oxidative damage. Inflammation is a natural immune reaction to injury or infection, but persistent inflammation can cause arthritis, diabetes, and cardiovascular disease. Anti-inflammatory drugs minimize inflammation and damage.

Methods:
The extracts were evaluated for their antioxidant activity utilizing in vitro tests, specifically the DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities. The anti-inflammatory capacity was assessed using in vitro measurements of cyclooxygenase (COX) enzyme inhibition and reduction of pro-inflammatory cytokines.
Results:
Significant antioxidant and anti-inflammatory effects were observed in both aqueous and methanolic extracts of Annona muricata. Nevertheless, the methanolic extract demonstrated a higher ability to scavenge free radicals and a stronger suppression of inflammatory mediators in comparison to the aqueous extract.
Conclusion:
The results indicate that the methanolic extract of Annona muricata contains a higher concentration of bioactive chemicals that are responsible for its strong antioxidant and anti-inflammatory properties. This study emphasizes the potential of Annona muricata, namely its methanolic extract, as a natural reservoir of medicinal substances for illnesses associated with oxidative stress and inflammation.

Additional investigation is required to separate and determine the particular compounds accountable for these actions and to examine their mechanisms of action