Formulation and Evaluation of PVA Loaded Nano Fibrous in Situ Gel.

Abstract

The comprehensive Preformulation study conducted on Bovine Insulin-loaded nanofibers involved an array of analytical techniques, each shedding light on critical aspects of the formulation's composition, stability, and performance. Fourier Transform Infrared (FTIR) spectroscopy revealed distinct peaks characteristic of Bovine Insulin, chitosan, sodium alginate, and other excipients, affirming the purity and compatibility of the components within the nanofibers. Differential Scanning Calorimetry (DSC) provided insights into the thermal behavior of Bovine Insulin and its interactions with polymers, crucial for understanding formulation stability. X-ray Diffraction (XRD) analysis highlighted a shift towards amorphous nature in Bovine Insulin powder when incorporated into nanofibers, indicating enhanced solubility. Additionally, determination of thiol groups elucidated the formation of bonds between chitosan and sodium alginate, contributing to the structural integrity of the nanofibers. Characterization studies, including SEM imaging, rheological analysis, and evaluation of spreadability and extrudability, provided valuable insights into the physical properties and applicability of the nanofiber gel, culminating in the identification of an optimized formulation, NGA5, exhibiting superior drug content uniformity, viscosity, and extrudability, thus holding promise for effective nasal delivery of Insulin.