Development And Validation Of HPLC Stability Indicating Assay Method For Trimethoprim In Bulk Drug And Tablets

Abstract

A Simple, picky, accurate, provident reverse phase high performance liquid chromatography (RP-HPLC) was developed for estimation of Trimethoprim in pharmaceutical phrasings. Chromatographic separation achieved on a Inertsil C₈ (100 x 4.6μ, 5μ) 250×4.6 mm i.d.) with mobile phase containing acetonitrile and potassium dihydrogen phosphate buffer [pH~ 6.5] in the ratio 35:65 v/v. The flow rate was 1.0 mL/min and effluent was covered at 250 nm. The retention time was 5.70 min. The tactic was validated in terms of linearity, delicacy and perfection. The linearity plant to be direct over 2.5 – 12.5 μg/mL. The limit of discovery and limit of quantification were plant to be 0.0066μg/mL and 0.022μg/mL respectively. The proposed method was successfully used to determine the medicine content of retailed phrasings.