Simultaneous Estimation of Cabotegravir and Rilpivirine in Combined Formulation by RP-HPLC Method

Abstract

For the simultaneous estimate of Rilpivirine and Cabotegravir in pharmaceutical dosage form, Rilpivirine and Cabotegravir were quantified in active pharmaceutical ingredients using a high-performance liquid chromatographic technology that was created and verified. Agilent C18 150 × 4.6 mm, 5µm Column was applied to run the Chromatographic technique. At a flow rate of 1.0 ml/min and mobile phase made up of Buffer of AmmoniumFormate: Acetonitrile in the proportion 70:30 was pumped through the column. 30°C was kept as the temperature. The obtained optimized wavelength was 257 nm. The retention times of rilpivirine and Cabotegravir were determined to be 2.322 minutes and 2.904 minutes respectively, Recovery was 99.84% and 100.14%, respectively of Rilpivirine and cabotegravir's and LOD and LOQ values through Regression equations yielded was 0.19, 0.59, and 0.20, 0.61, respectively.Rilpivirine’s regression equation is y = 6645.3x + 720.01 and Cabotegravir’sis y = 6696x + 327.18. As a result of shorter retention times and shorter run times, the method was created to be straightforward and cost-effective, and it may be used for routine Quality Control Tests in Industries.