Study the Harmful Effects of Indomethacin in Liver of Male Albino Rats and Role of the Nano-Extract of Cardia Myxa Fruits in Preventing

Abstract

The current study was conducted in the animal house-College of Science / University of Kufa. The study aimed to reveal the protective role of nano-extract of the fruits of the cardia myxa plant on the histological composition of the kidneys of male white rats treated with indomethacin, their ages ranged between (8-10) weeks and their weights ranged between (200-250) gm, then the animals were divided into (8) groups, each group contained (5) male rats and were as follows: the first group represented the control group which was dosed with physiological saline only, while the the second group is the group treated with indomethacin at a concentration of 0.143 mg/kg, while the third and fourth groups were the group treated with nanoextract at two concentrations (20 and 30 mg/kg, respectively, While the fifth group was the group that dosed zinc oxide, Zno, at a concentration of (1 mg),  and the sixth and seventh group represented the group treated with the nanoextract at two concentrations (20 and 30 mg/kg) + the drug Indomethacin at a concentration of (0.143) mg/kg respectively, while the eighth group was treated with the plant extract of the fruits of the Panemer plant, 500 mg/kg + indomethacin  at a concentration of (0.143) mg/kg respectively.  Male rats were dosed in all groups for a period of (40 days) at the rate of one dose per day through a gastric dosing tube, after which the animals were sacrificed and microscopic sections of the liver were made for histological study.  The results showed in the control group the normal tissue of the liver, as it was the normal histological structure  of the liver, while the results for the drug group showed the presence of pathological changes in the liver tissue represented by the presence of necrosis and severe hemorrhage, while the histological results of the two nanoextract groups with two concentrations (20 and 30 mg / kg), respectively It showed the normal shape of the hepatocytes and the regularity of the hepatic cords. As for the results of the group treated with zinc oxide (Zno), it revealed harmful effects to the liver tissue, such as severe necrosis of the cells and the expansion of sinusoids, while the results of the sixth and seventh groups, treated with two concentrations of nano-extract + indomethacin drug, showed that the structure of the liver was close to normal tissue with little change in hepatocytes and regular hepatic cords, as for the results of the eighth group, the plant extract group of the fruits of the panamer plant of  C.myxa  at a concentration of 500 mg / kg + the drug at a concentration of (0.143) mg / kg, it was observed that slight changes occurred in the liver tissue, such as necrosis in some liver cells and degeneration of some of them.